Assistant Laboratory Animal Technician (ALAT) Practice Exam

Which safety cabinet is most commonly utilized for handling low to moderate risk biological materials?

1. Class I BSC

2. Class II BSC

3. Class III BSC

4. Class IV BSC

The correct answer is: Class II BSC

The Class II Biological Safety Cabinet (BSC) is most commonly utilized for handling low to moderate risk biological materials due to its design that provides both personnel and environmental protection. Class II BSCs offer a controlled airflow, which creates a sterile environment for the safe handling of materials that may pose a risk of infection, such as research involving certain pathogens and cell cultures. These cabinets are designed to maintain a constant flow of air through the work area, which helps to contain aerosols and protects both the user and the work being conducted. Their HEPA (High-Efficiency Particulate Air) filters ensure that any airborne contaminants are filtered out, providing a safe working environment. In contrast, other types of safety cabinets serve different purposes or are intended for different risk levels. Class I BSCs, while providing personnel protection, do not protect the product inside the cabinet, making them less suitable for materials that require a sterile environment. Class III BSCs are designed for handling high-risk biological agents and provide the highest level of protection, typically used in biosafety laboratories. Class IV BSCs are used for working with the most dangerous and exotic pathogens that pose a high risk to health and the environment, requiring additional safety measures beyond those provided by