Assistant Laboratory Animal Technician (ALAT) Practice Exam

Which method is considered very common for euthanizing mice?

1. Physical restraint

2. CO2 asphyxiation

3. Injection of anesthetics

4. Decapitation

The correct answer is: CO2 asphyxiation

The method of euthanizing mice that is considered very common is the use of CO2 asphyxiation. This technique is widely accepted and utilized because it leads to a rapid loss of consciousness followed by death, making it a humane option when performed correctly. Carbon dioxide is administered in a controlled manner, typically through a chamber that allows for the gradual increase of CO2 concentration. This gradual induction minimizes distress and pain for the animal. The effectiveness and efficiency of CO2 asphyxiation have led to its recommendation by various guidelines and organizations involved in animal care and research, emphasizing humane endpoints in scientific procedures. This method is also practical in terms of ease of use and availability, making it a standard practice in many laboratory settings.