Assistant Laboratory Animal Technician (ALAT) Practice Exam

What should be monitored to ensure the safe operation of an autoclave?

1. Ambient temperature only

2. Gas levels only

3. Pressure and temperature gauges

4. Humidity and noise levels

The correct answer is: Pressure and temperature gauges

Monitoring pressure and temperature gauges is essential for the safe operation of an autoclave because these parameters ensure that the sterilization process is effective. The autoclave uses steam under pressure to kill microorganisms, including bacteria, viruses, and spores. If the temperature is not reached, or if the pressure is insufficient, the sterilization process may fail, resulting in the survival of pathogens. This can pose a significant risk in laboratory settings where contamination could lead to compromised experiments or spread of infectious agents. Therefore, consistently checking the pressure and temperature gauges confirms that the autoclave is operating within the required specifications for effective sterilization. While other factors like ambient conditions or gas levels can affect operations, they are not central to the autoclave's sterilization process in the same way that pressure and temperature are. Monitoring these gauges not only ensures compliance with health and safety regulations but also maintains the integrity of laboratory practices.