Assistant Laboratory Animal Technician (ALAT) Practice Exam

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What is the primary function of an autoclave?

  1. To weight materials

  2. To sterilize instruments and equipment

  3. To store hazardous materials

  4. To analyze chemical properties

The correct answer is: To sterilize instruments and equipment

The primary function of an autoclave is to sterilize instruments and equipment. An autoclave uses steam under pressure to achieve high temperatures that kill microorganisms, including bacteria, viruses, fungi, and spores. This sterilization process is critical in laboratory settings, especially when preparing instruments and materials for procedures that require a sterile environment, such as surgeries or experiments involving live animals. The steam's high temperature efficiently penetrates materials, ensuring that they are free from any potential contaminants. In contrast, weighing materials involves using scales or balances, which do not relate to the function of an autoclave. Storing hazardous materials requires specific containment solutions, not the use of an autoclave. Analyzing chemical properties typically involves different types of laboratory equipment designed for chemical and physical analyses, rather than sterilization processes. Therefore, the role of the autoclave is distinct and essential for maintaining cleanliness and safety in laboratory and medical environments.